Research Use Cases for Charcoal-Stripped Human Serum
The research paper titled:
Serum Estrogen Metabolites and Systolic Blood Pressure Among Middle-Aged and Older Women and Men
By Christopher M. Masi, Louise C. Hawkley, Xia Xu, Timothy D. Veenstra, John T. Cacioppo
- Full paper can be found here.
This study explores the potential link between endogenous estrogens and systolic blood pressure (SBP) in middle-aged and older adults. The study focuses on serum concentrations of 17β-estradiol (E2) and its metabolites, particularly 16α-hydroxyestrone (16α-OHE1) and 16-ketoestradiol (ketoE2), to investigate their associations with SBP.
The research, conducted through a cross-sectional study design, analyzed data from a population-based sample of 98 adults aged 55 to 69 in Cook County, Illinois. Serum samples were examined for E2 and 14 estrogen metabolites using mass spectrometry. SBP was measured, and demographic and health history information was collected through questionnaires.
Findings:
The key findings of the study indicate an inverse relationship between SBP and both natural log (ln) 16α-OHE1 and ln 16-ketoE2 among postmenopausal women but not among men. Multivariate analysis, adjusting for various factors such as age, race, ethnicity, body mass index (BMI), and use of cardiovascular medications, confirmed the negative association of ln 16α-OHE1 and ln 16-ketoE2 with SBP in postmenopausal women.
Calibration Standards:
The importance of charcoal-stripped human serum in the study is particularly in the preparation of calibration standards and quality control samples for estrogen metabolite assays. The primary purpose of charcoal stripping is to eliminate endogenous hormones from the serum sample, creating a hormone-depleted medium for subsequent analyses.
In studies focusing on estrogen metabolites, charcoal stripping ensures that the measured hormone levels are reflective of exogenously introduced substances rather than being influenced by the hormones naturally present in the serum. Calibration standards are used to create a reference curve for quantifying the concentrations of estrogen metabolites in the serum samples.
Conclusion:
The article emphasizes the significance of understanding the relationship between serum estrogen concentrations, particularly 16α-OHE1 and 16-ketoE2, and vascular health among postmenopausal women.
In conclusion, the study suggests that 16α-OHE1 and 16-ketoE2 may play a crucial role in vascular health among postmenopausal women, influencing SBP. The findings contribute to the understanding of the potential cardiovascular effects of specific estrogen metabolites and underscore the importance of further research to explore causation and potential therapeutic implications.
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References:
Christopher M. Masi, Louise C. Hawkley, Xia Xu, Timothy D. Veenstra, John T. Cacioppo, Serum Estrogen Metabolites and Systolic Blood Pressure Among Middle-Aged and Older Women and Men, American Journal of Hypertension, Volume 22, Issue 11, November 2009, Pages 1148–1153, https://doi.org/10.1038/ajh.2009.155