The importance of charcoal-stripped human serum in the study is particularly in the preparation of calibration standards and quality control samples for estrogen metabolite assays. The primary purpose of charcoal stripping is to eliminate endogenous hormones from the serum sample, creating a hormone-depleted medium for subsequent analyses.

Hormone-free human serum serves as a baseline, enabling researchers to isolate the specific effects of their experimental variables with greater precision. By eliminating the confounding influences of hormones, scientists can investigate cellular responses, evaluate drug efficacy, and gain valuable insights into various biological pathways.