MSG4000: Mass Spect Gold Human Serum, Ultra-Low Hormones & Steroids

The study shows that while Cyp26a1, a retinoic acid hydroxylase, has a minimal impact on maintaining normal postnatal vitamin A levels, it is essential for clearing exogenously administered all‐trans retinoic acid (atRA). Disruption of Cyp26a1 leads to impaired atRA clearance, highlighting its protective role against retinoid toxicity.

MSG4000 is used as a specialized matrix reagent during the extraction and quantification of retinoids. It provides a stable, serum-like environment that ensures precise and reproducible measurement of atRA and its metabolites, which is critical for assessing the metabolic role of Cyp26a1.

For a detailed look at the study's findings and the specific use of MSG4000 serum, you can read the full article at Journal of Biological Chemistry.

This study investigates the relationship between Cytochrome P450 2D6 (CYP2D6) enzyme activity and plasma bile acid concentrations during and after pregnancy. The study found that plasma concentrations of taurocholic acid were positively correlated with the activity of CYP2D6, implying that variations in bile acid levels during pregnancy may influence the enzyme's activity.

Golden West's MSG4000 serum was used to create calibration standards and quality control samples for measuring bile acids, facilitating the analysis of their systemic concentrations in pregnant women. The study provides evidence that alterations in the plasma bile acid metabolome during pregnancy may affect CYP2D6 activity, which is important for the metabolism of many drugs.

For a detailed look at the study's findings and the specific use of MSG4000 serum, you can read the full article at ASPET.

The study presents a mass spectrometry-based method for precise measurement of thyroglobulin (Tg) in serum, offering a more sensitive alternative to traditional immunoassays. Using a monoclonal antibody for peptide purification and DMSO for enhanced detection, the method achieved a lower limit of quantification of 0.15 ng/mL.

The MSG4000 human serum pool was utilized to create a "Husky Ref" reference material, ensuring assay consistency across laboratories. This advancement aims to standardize Tg testing, particularly for patients with interfering autoantibodies.

For a detailed look at the study's findings and the specific use of MSG4000 serum, you can read the full article at ScienceDirect.

The research paper describes a clinical research method using UPLC-MS/MS for analyzing serum estrogens, focusing on estradiol (E2) and estrone (E1), without requiring derivatization and utilizing only 250 µL of sample.

MSG4000 stripped serum was employed as a surrogate matrix to prepare calibrator and quality control samples, which are key to the method's sensitivity and precision. With a lower limit of quantification of 3 pg/mL for E2 and 2 pg/mL for E1, the developed assay demonstrated excellent precision, accuracy, and linearity, effectively differentiating between a blank sample and one spiked with 1 pg/mL of E2.

The study highlights the method's capability to achieve robust results with minimal sample volume and without significant endogenous interference.

For a detailed look at the study's findings and the specific use of MSG4000 serum, you can read the full article at Waters.

The study aimed to compare the performance of four different clinically validated liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for testosterone determination by conducting interlaboratory comparisons using human serum samples.

The double charcoal-stripped human serum (MSG4000) was employed as a matrix for calibration and quality control in these assays. The study aimed to assess how well different LC-MS/MS methods correlate and harmonize in testosterone quantification, which is important for ensuring accurate and reliable clinical testing across different laboratories.

For a detailed look at the study's findings and the specific use of MSG4000 serum, you can read the full article at PMC.

The research paper investigates plasma retinoid concentrations in pregnant women and compares them with levels ≥3 months postpartum. The study found that plasma retinol, retinol binding protein 4 (RBP4), transthyretin, and albumin concentrations decreased during pregnancy but returned to baseline postpartum.

Golden West’s Double Charcoal Stripped Serum (MSG4000) was used as a surrogate matrix for creating calibration and quality control (QC) samples for the quantification of estrogens E2 and E1. This facilitated the assay's capability to distinguish between E2 levels in blank samples and low concentration spiked samples, thus demonstrating significant changes in maternal retinoid concentrations due to pregnancy.

For a detailed look at the study's findings and the specific use of MSG4000 serum, you can read the full article at MDPI.

This study examines the variations in retinoid concentrations across pregnancy and after childbirth. Using liquid chromatography-tandem mass spectrometry, the study observed significant fluctuations in the levels of retinoids like all-trans-retinoic acid (atRA), 13-cis-retinoic acid (13-cis RA), and their oxidative metabolites. The researchers discovered a decrease in 13-cis RA across pregnancy with an increase in levels post-delivery and an increase in atRA concentrations mid-pregnancy.

Golden West's MSG4000 serum was utilized as the matrix for the calibration standards and as a surrogate matrix for quality control samples in the quantification process of retinoids. This study provides new insights into the changes in retinoid homeostasis in pregnant women.

For a detailed look at the study's findings and the specific use of MSG4000 serum, you can read the full article at PLoS One